Reverse Genetics Systems for Emerging and Re-Emerging Swine Coronaviruses and Applications

نویسندگان

چکیده

Emerging and re-emerging swine coronaviruses (CoVs), including porcine epidemic diarrhea virus (PEDV), deltacoronavirus (PDCoV), acute syndrome-CoV (SADS-CoV), cause severe in neonatal piglets, CoV infection is associated with significant economic losses for the industry worldwide. Reverse genetics systems realize manipulation of RNA genome facilitate development new vaccines. Thus far, five reverse approaches have been successfully applied to engineer genome: targeted recombination, vitro ligation, bacterial artificial chromosome-based vaccinia -based yeast-based method. This review summarizes advantages limitations these approaches; it also discusses latest research progress terms their use virus-related pathogenesis elucidation, vaccine candidate development, antiviral drug screening, replication mechanism determination.

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ژورنال

عنوان ژورنال: Viruses

سال: 2023

ISSN: ['1999-4915']

DOI: https://doi.org/10.3390/v15102003